peripheral blood mononuclear cell isolation kit  (Beijing Solarbio Science)

Journal: International Dental Journal

Article Title: Obesity as a Determinant of Periodontal Therapy Outcomes: Insights on Oxidative and Endoplasmic Reticulum Stress Pathways

doi: 10.1016/j.identj.2026.109472

Figure Lengend Snippet: Oxidative stress markers in the study population, stratified by obesity presence, before and after non-surgical periodontal treatment. Total ROS (A and G), cytosolic superoxide (B and H) and mitochondrial superoxide (C and I) in leukocytes, and protein levels of SOD1 (D and J) in PBMCs normalized to the loading control actin with corresponding WB images (E), and serum total antioxidant capacity (F and K). Data are presented as box and whisker plots and were compared using two-factor analysis of variance (ANOVA) followed by post hoc tests. ** P < .01 when comparing baseline vs 12 wk. # P < .05 when comparing groups with vs without obesity. DCFH, diclorodihidrofluoresceína; dHE, dihydroethidium; MitoSOX, Mitochondrial Superoxide Indicator; ns, not significant; PBMCs, peripheral blood mononuclear cells; ROS, reactive oxygen species; SOD1, superoxide dismutase 1.

Article Snippet: Peripheral blood mononuclear cells (PBMCs) comprising monocytes and lymphocytes were isolated from EDTA-collected blood using the MACSprep PBMC Isolation Kit (Miltenyi Biotec, Teterow, Germany), and 5 × 106 cells were used for protein extraction, quantification, and Western blot (WB) analysis.

Techniques: Control, Whisker Assay

Journal: International Dental Journal

Article Title: Obesity as a Determinant of Periodontal Therapy Outcomes: Insights on Oxidative and Endoplasmic Reticulum Stress Pathways

doi: 10.1016/j.identj.2026.109472

Figure Lengend Snippet: Evaluation of UPR markers in PBMCs from study population, stratified by presence of obesity, before and after non-surgical periodontal treatment. Relative protein expression of GRP78 (A and G), ATF6 (B and H), CHOP (C and I), IRE1α (D and J), p-eIF2α (E and K), relative mRNA expression of sXBP1 (F and L) in PBMCs normalized to the loading control actin and representative WB images (M). Data are presented as box and whisker plots and were compared using two-factor analysis of variance (ANOVA) followed by post hoc tests. * P < .05; ** P < .01 when comparing baseline vs 12 wk. ATF6, activating transcription factor 6; BMI, body mass index; CHOP, C/EBP homologous protein; GRP78, Glucose-regulated protein 78; IRE1α, Inositol requiring enzyme 1α; PBMCs, peripheral blood mononuclear cells; p-eIF2α, Phosphorylated eukaryotic translation initiation factor 2 subunit; sXBP1 , spliced Xbox binding protein 1 gene.

Article Snippet: Peripheral blood mononuclear cells (PBMCs) comprising monocytes and lymphocytes were isolated from EDTA-collected blood using the MACSprep PBMC Isolation Kit (Miltenyi Biotec, Teterow, Germany), and 5 × 106 cells were used for protein extraction, quantification, and Western blot (WB) analysis.

Techniques: Expressing, Control, Whisker Assay, Binding Assay

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: A needle-free intradermal PEDV S protein vaccine elicits protective lactogenic immunity in piglets

doi: 10.3389/fcimb.2026.1720440

Figure Lengend Snippet: Vaccine-induced humoral and cellular immune responses in piglets. (A) Serum PEDV-specific IgG antibody titers determined by 10-fold serial dilution ELISA. The dotted line represents a serum ratio of 0.8, above which samples were considered positive. (B) PBMC proliferation-associated metabolic activity measured by CCK-8 assay. PBMCs were isolated at day 14 post-boost, cultured in RPMI-1640 medium, and stimulated with PEDV-S protein (10 µg/mL) for 72 h. (C) IFN-γ levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation. (D) IL-4 levels in PBMC culture supernatants quantified by ELISA following PEDV-S protein stimulation Data are shown as mean ± SD (n = 6 per group). Statistical significance: ***p < 0.001.

Article Snippet: At day 14 post-boost, peripheral blood mononuclear cells (PBMCs) were isolated using a porcine peripheral blood mononuclear cell isolation kit (Solarbio, China) according to the manufacturer’s instructions.

Techniques: Serial Dilution, Enzyme-linked Immunosorbent Assay, Activity Assay, CCK-8 Assay, Isolation, Cell Culture