Journal: Frontiers in Immunology

Article Title: Reorganization and functional divergence of the CD4+ memory T cell compartment in hidradenitis suppurativa

doi: 10.3389/fimmu.2026.1831664

Figure Lengend Snippet: Expansion of central memory T cells (T CM ) in HS skin. Flow cytometric analysis of total CD4+ and CD8+ T cell subsets in (A) the skin and (B) peripheral blood. Frequency of naïve and memory subsets within the CD4+ and CD8+ compartments are shown for (C) skin and (D) peripheral blood. Each dot represents an individual donor (Healthy, diamonds; HS, circles); bars indicate the mean frequency of the population relative to the indicated gate ± standard deviation (SD). Healthy PBMCs, n = 8; Healthy skin, n = 5; HS skin and PBMCs matched samples ( n = 5). Detailed gating strategies for T cell memory subsets (Naïve, T CM , T EM and T RM ) are provided in Supplementary Figure 1 . * P < 0.05, ** P < 0.01 and *** P < 0.001 (Two-way ANOVA with Šídák’s multiple comparisons test).

Article Snippet: Peripheral blood mononuclear cells (PBMCs) from blood were isolated with StraightFrom ® Whole Blood PBMC Isolation Kit, human (130-126-359, Miltenyi Biotec), and resuspended in the same culture medium as skin cells.

Techniques: Standard Deviation

Journal: Frontiers in Immunology

Article Title: Reorganization and functional divergence of the CD4+ memory T cell compartment in hidradenitis suppurativa

doi: 10.3389/fimmu.2026.1831664

Figure Lengend Snippet: Heightened pro-inflammatory potential of skin vs peripheral blood CD4+ memory T cells. Frequencies of cytokine-expressing memory T cell subsets from matched skin and PBMC samples ( n = 4). Bars represent the mean percentage (± SD) of cytokine-expressing memory T cells vs parent, under unstimulated baseline conditions (“Unstim”, blue bars/circles) and following stimulation with anti-CD3/anti-CD28 coated beads (“aCD3/aCD28”, red bars/triangles). Data are shown for TNF-α-producing CD4+ T EM (A) and T CM (D) cells, IFN-γ-producing CD4+ T EM (B) and T CM (E) , IL-17A-producing CD4+ T EM (C) and T CM (F) . Statistical differences between skin and PBMCs and stimulation condition were evaluated by 2-way repeated measures ANOVA and Fisher’s LSD test (* P < 0.05 and ** P < 0.01).

Article Snippet: Peripheral blood mononuclear cells (PBMCs) from blood were isolated with StraightFrom ® Whole Blood PBMC Isolation Kit, human (130-126-359, Miltenyi Biotec), and resuspended in the same culture medium as skin cells.

Techniques: Expressing

Journal: Journal of Medicinal Chemistry

Article Title: Biocompatible Glycoconjugation Enables Sensitive In Vivo Cell Tracking by PET/CT

doi: 10.1021/acs.jmedchem.6c00538

Figure Lengend Snippet: Region of Interest (ROI) analysis of PET/CT imaging. A) [ 89 Zr]­Zr–U937 cells. B) [ 89 Zr]­Zr-PBMCs. C) [ 89 Zr]­Zr-NHP-Neutrophils. D) [ 89 Zr]­Zr-NHP-T cells.

Article Snippet: Human primary nonmobilized PBMCs were obtained from healthy donor peripheral blood using an institutional review board (IRB)-approved protocol (2017–0170) and isolated using the MACSprep PBMC Isolation Kit (Miltenyi Biotec, San Jose, CA) without density gradient centrifugation.

Techniques: Positron Emission Tomography-Computed Tomography, Imaging

Journal: bioRxiv

Article Title: Extrahepatic, cell-specific delivery of LNPs through competitive inhibition of ApoE-mediated uptake

doi: 10.64898/2026.05.29.727812

Figure Lengend Snippet: (A) Flow cytometric analysis showing hCD3-NP transfection of human pan T cells within unactivated PBMC cultures using MC3-DOPE-LP LNPs. Coating with hCD3-NP increases the transfection (percent EGFP+) of both CD4+ and CD8+ T cells. (B) MFI of EGFP and (C) DiD following transfection of unactivated human PBMCs. While untargeted MC3-DOPE-LP LNPs exhibit pronounced off-target uptake by monocytes, hCD3-NP titration led to decreased CD14+ uptake and increased CD4+ and CD8+ T cell uptake. (D) Comparison of the percentage of EGFP+ cells in unactivated human PBMC mixtures treated with MC3-versus SM102-based LNPs ± 500 nM hCD3-NP. (E) EGFP and (F) DiD MFI data comparing the targeting profiles of the MC3 and SM-102 formulations ± 500 nM hCD3-NP. EGFP, enhanced green fluorescent protein; hCD3, human CD3; LNP, lipid nanoparticle; MFI, median fluorescence intensity; NP, NanoPilot; PBS, phosphate-buffered saline.

Article Snippet: Human PBMCs (received from donors from NHS Blood and Transplant) were isolated from buffy coats using the StraightFrom Buffy Coat PBMC Isolation Kit (human; Miltenyi Biotec) according to the manufacturer’s instructions.

Techniques: Transfection, Titration, Comparison, Fluorescence, Saline